|
KMID : 0379120170450040386
|
|
Korean Journal of Mycology
2017 Volume.45 No. 4 p.386 ~ p.393
|
|
|
Phylogenetic Analysis of Downy Mildew Caused by Peronospora destructor and a Method of Detection by PCR
|
|
Back Chang-Gi
Hwang Sun-Kyung
Park Mi-Jeong
Kwon Young-Seok
Jung Hee-Young
Park Jong-Han
|
|
|
Abstract
|
|
|
|
Onion downy mildew, caused by, is a major disease in onion cultivation areas in Korea. The causal fungi were collected and analyzed based on sequence similarity and molecular phylogenetic relationships of multi-gene sequences, including the internal transcribed spacer (ITS) region. All isolates from Changnyeong-gun, Hamyang-gun, and Hapcheon-gun in Gyeongnam province, and Muan-gun, Haenam-gun, and Sinan-gun in Jeonnam province were identical in the four types of gene sequences, indicating they were genetically the same strains. In this study, a PCR method was developed based on the ITS gene sequences to amplify the specific DNA fragment for P. destructor only. The detection limit of was total genomic DNA of the P. destructor and the plant 0.7 ng/μL. Therefore, the developed PCR method could be used to detect P. destructor effectively from symptomless onion leaves.
|
|
|
KEYWORD
|
|
|
Detection, Downy Mildew, Onion, PCR, Peronospora destructor
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|